We work on the characterization and functional analysis of genes. Our team members work on the characterization of virulence genes in human pathogenic bacteria. Also conduct research on the semi-quantitative determination of gene expression. The techniques used here are rapd, rflp, aflp, multiplex, reverse transcriptase pcr.
Metagenomic approach is one of the powerful tools to figure out the microbial diversity as well as to study the ecology of microbes in different environment.We have standardized the techniques to extract genetic material directly from environmental samples (soil, water and food,). We work on the rapid detection of environmental pathogens by amplifying the genetic material extracted from various sources. Proteomics analysis of complete set of proteins of microbial and plant sources are currently undertaken. Electrophoretic separation, blotting of proteins, in-gel activity analysis of enzymes are few among the techniques used.
Microbiology / Bacteriology research group works on the phenotypic and genotypic characterization of human pathogens. The team has developed methods to extract DNA from clinical specimens such as blood, urine and sputum. Also conduct research on the drug resistance and plasmids, including transferable plasmids, transformation of plasmids, plasmid profiling, conjugation and plasmid curing. We also work with bacterial virulence genes.
Environmental samples are processed and the microbial load (bacteria, fungi and actinomycetes) are enumerated using conventional methods. We are familiar with techniques that used for the bioremediation of contaminated soil.
We undertake research about the biofertilizers, inoculums development and optimization of conditions for its better activity. Also work on the plant microbe interaction. The research team has involved in developing inoculums that can enhance plant growth and yield.
Microorganisms are identified on the basis of 16s rRNA. The conventional biochemical methods have become non-reliable in identifying microbes. In our laboratory we undertake research work to identify the microbes at species level based on the determination of 16s rRNA sequence.
Plant phytochemical extraction and its qualitative analysis is provided Extraction of Essential phyto products and oils Proteins and genes related to biotic and abiotic stress in crop plants are characterized. Plant leaf and seed proteins with and without stress challenging and viral infection related proteins are currently studied. Antioxidant enzymes related to stress tolerance are being characterized. We work with cultivated wild types and transgenic plants.
We study the bioremediation by microorganisms and biomass utilization by both bacteria and fungi. Our team conducts experiments on the biodegradation of xenobiotics such as petroleum hydrocarbons and pesticides. We have developed a microbial consortium to effectively degrade the toxic substances. We also work with other agro-based organic materials and industrial effluents.
We carry out research on development of bioformulations like biosurfactants, biopolymers, biopesticides and bioinoculants. We analyze the unique features of the beneficial microbes at the molecular level for the biotechnology exploitation.
Our scientists support student projects and training involving biological databases, structural database, small molecules, drug databank, sequence alignment, evolutionary and phylogenetic analysis. Genome and proteome mining, modeling, gene prediction, gene annonation are also undertaken. Research and analysis on computer aided drug design, docking analysis, target analysis from metabolic pathway are carried out